Figure 4

Activation of Notch1 in adipocytes inhibits browning and glucose metabolism. (a) Relative expression of N1ICD and its target genes in WAT. (b,c) Blood glucose concentrations during IP-GTT (b, n = 6) and IP-ITT (c) performed on 5–8 week-old WT and Adipoq/N1ICD mice. (d–f) O₂ consumption (d), CO₂ production (e), and heat production (f). (g) Rectal temperature, n = 5. (h) Relative expression of BAT and mitochondria marker genes in IngWAT (top) and BAT (bottom). (i) Western blot of IngWAT. (j) H&E staining of BAT section, scale bar, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001. Data are means ± SEM. n = 4 pairs of mice unless otherwise indicated.