Figure 3

aNotch1 mice were resistant to HFD-induced obesity. (a,b) Growth curve (a) and energy intake assay (b, n = 4) of WT and aNotch1 fed with HFD. (c) Blood glucose concentrations during IP-GTT after glucose injection into fasted mice. (d) Blood glucose concentrations during IP-ITT after injecting insulin into fasted mice. (e) Gene expression assay of Notch receptors, Notch targets and Lep in WAT from 3 weeks’ standard diet (SD) and HFD fed mice, n = 8. (f) Representative result of western blot for Notch activity of sample as in e. *P < 0.05, **P < 0.01, ***P < 0.001. Data are means ± SEM. n = 5 pairs of mice unless otherwise indicated.